Form: 10-K

Annual report pursuant to Section 13 and 15(d)

February 28, 2022

 EXHIBIT 21

SUBSIDIARIES OF CRYOPORT, INC.

AND JURISDICTION OF INCORPORATION OR ORGANIZATION

Cryogene, Inc.

Texas

Cryoport Systems, LLC

California

MVE Biological Solutions US, LLC

Delaware

Cryoport Netherlands BV

The Netherlands

Cryoport Japan GK

Japan

Cryoport UK Limited

United Kingdom

MVE Biological Solutions Australia Pty Limited

Australia

MVE Biological Solutions Germany GmbH

Germany

MVE Cryogenic Biological Equipment (Chengdu) Co., Ltd.

China

Advanced Therapy Logistics and Solutions, SAS

France

Cryo International SA

France

Cryo Express SA

France

Cryo Express SP. ZO.O.

Poland

Cryo Express GmbH

Germany

Cryo Express Pty. Ltd.

Australia

SPL Services Limited

United Kingdom

CryoPDP Global Services, Unipessoal LDA

Portugal

CryoPDP KK

Japan

I.C.S. Dry-Ice Express B.V.

The Netherlands

PDP Courier Services Limited

United Kingdom

PDP Courier Services (USA), Inc.

Delaware

PDP Couriers (Singapore) PTE. LTD

Singapore

PDP Couriers Korea Co., Ltd.

South Korea

PDP Life Science Logistics India Private Limited

India

Critical Transport Solutions Australia Pty. Ltd.

Australia

2GTR

Belgium