1

Item 1.01 Entry into a Material Definitive Agreement

On October 14, 2010, CryoPort, Inc. (the “Registrant”) entered into definitive agreements in connection with the second close of a private placement of its securities to certain institutional and accredited investors (the “Investors”) that commenced in August 2010. In connection with the second closing of the private placement, the Registrant received aggregate gross proceeds of $583,000 (approximately $539,690 after cash offering expenses) pursuant to the Securities Purchase Agreements between the Registrant and each Investor (the “Purchase Agreements”) and the Registration Rights Agreement among the Registrant, Emergent Financial Group, Inc., and the Investors (the “Registration Rights Agreement”). The Registrant intends to use the proceeds for working capital purposes.

Pursuant to the Purchase Agreements executed in connection with the second closing, the Investors purchased an aggregate of 832,868 units (the “Units”), with each Unit consisting of (i) one share of common stock of the Registrant (“Common Stock”), and (ii) one warrant to purchase one share of Common Stock at an exercise price of $0.77 per share. The warrants are immediately exercisable and have a term of five years.

With respect to the first and second closings of the private placement, the Registrant raised net proceeds of approximately $3.6 million.

Emergent Financial Group, Inc. served as the Registrant’s placement agent in connection with the second closing of the private placement and received a customary fee of 7% of the aggregate gross proceeds received from the Investors in the second closing, plus reimbursement of expenses in the amount of $2,500, and was issued a warrant to purchase 116,602 shares of Common Stock, at an exercise price of $0.77 per share.

Pursuant to the Registration Rights Agreement, on October 19, 2010, the Registrant filed a registration statement on Form S-1 with the Securities and Exchange Commission registering the resale of the shares of Common Stock issued to the Investors in both closings and the shares of Common Stock underlying the warrants issued to the Investors and placement agents in both closings.

The foregoing summary of the terms and conditions of the Purchase Agreements, the Registration Rights Agreement and the warrants does not purport to be complete and is qualified in its entirety by reference to the full text of each of the aforementioned documents which were filed as exhibits to the above mentioned registration statement on Form S-1.

Item 3.02 Recent Sale of Unregistered Securities

The sale and issuance of the Units, the Common Stock, and the warrants was completed in accordance with the exemption provided by Rule 505 and/or Rule 506 of Regulation D of the Securities Act of 1933, as amended (the “Securities Act”), and/or Section 4(2) of the Securities Act.

2

SIGNATURES

Pursuant to the requirements of the Securities Exchange Act of 1934, the Registrant has duly caused this report to be signed on its behalf by the undersigned hereunto duly authorized.

3